The primary mission of the Cremins lab is to have a lasting impact on our understanding of the genetic and epigenetic mechanisms regulating mammalian brain development and function. We map neural epigenomes in three-dimensions using quantitative, systems level, genome-wide technologies. We also perturb 3-D epigenomes by developing and applying state-of-the art genetic engineering (CRISPR/Cas9, 3D optoepigenetic) technologies. We use human induced pluripotent stem cell differentiation and organoids as our models system(s) to understand the brain. Our long-term scientific goal is to make discoveries that inspire therapeutic strategies to prevent, reverse, or treat neurological disease states. We seek to create a body of work known for its veracity, scientific excellence, and creative insights. Our long-term mentoring goal is to develop the next generation of scientific leaders cross-trained in wet and dry lab approaches. We seek to create a positive, high energy environment with open, honest communication to empower individuals to discover and refine their purpose and grow into the best version of themselves.
To deliver on our primary mission, the Cremins lab pursues three central directions:
- Dissect the cause-and-effect relationship between the 3D Epigenome and synaptic plasticity using (i) electrophysiological & optogenetic stimulation of mouse hippocampal slices, (ii) human brain organoids, and (iii) behavioral mouse models of long-term memory formation
- Investigate when, how and why epigenetic mechanisms go awry during the onset and progression of neurodevelopmental and neurodegenerative disease states
- Develop tools to engineer 3-D Epigenomes for precise spatiotemporal control of gene expression states in healthy and diseased neurons